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2009-05-06 21:37 |
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Compared with the original plant M8 hydrogen sulphide, but is not obvious, and sulfur dioxide increase in beer yeast mutations M8 as the research object, by means of genetic engineering construct sulphite secretion of beer yeast engineering bacterium. Through the excessive expression in saccharomyces cerevisiae cytoplaic membrane coding of pump (S) "S U l p SSU1 gene, to improve of sulphite output. However, the researchers abroad for SO2 research focuses on the metabolic pathway through controlling sulfite several key enzyme gene expression, such as increasing the number of copies MET3 and MET14, make MET2 or MET10 gene or missing part of fracture, to improve the content of SO2 in beer, SSU1 for research. We will have different length of SSU1 gene promoter, construct and carrier recombination expression plaid pSU1 respectively, and the transformation of pSU2 YS58 saccharomyces cerevisiae laboratory to express the effect upon verification, anti-fuzzy for determination of sulfur dioxide and hydrogen sulphide cargoes, found the sulfur demand has increased the demand with the original, hydrogen sulfide (YS58) are all, and the two sons of the transformation of sulfur demand no difference, the length of the promoters of SSU1 gene expression, so choose good expression containing purpose gene expression of SSU1-2 pSU2 carrier into industrial beer yeast mutations M8, further research SSU1 gene expression in industrial beer yeast for so2 cargoes. |
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