| 愚者不惑也 |
2011-04-21 19:23 |
This is a brief selection of the many available TLC visualization reagents. Below each title is the type of
compounds or structure which can be detected with the specific reagent. When beginning work with these
reagents, acquired any MSDS (material safety data sheets) to see if there are any extra precautions needed in
safely using them.
Before spraying, plates should be well dried in the hood of residual solvents and components. Amines and
organic acids used in the mobile phases may adversely affect the visualization reaction being attempted. If
heating to remove these components is done, consideration should be given so that loss of components or their
decomposition is avoided (by lowering the temperature or using a shorter time in the oven).
Always spray any of these reagents onto plates in a well ventilated hood while wearing safety glasses. Also
apply moderate amounts to the plate so it always appears dull and flat (if it looks wet, you have sprayed too
much). You can always overspray to enhance the detection.
When information about the results of using the visualization reagents was available, this was put under each
reagent as ‘Results’. If not give, the user will have to do a few experiments to see what the results might be.
Always remember to look under normal light and also short and long wavelength UV light so as not to miss any
possibilities.
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